Both teams prevailed in their Liga Mx "Liguilla" semifinal playoffs on Sunday and head to the Apertura championship series Thursday in Tijuana and Sunday in Toluca.
Tijuana, which began in 2006 and only ascended to the Mexican first division in 2011, overcame a 2-0 loss in the first leg on Thursday. Richard Ruiz scored in the first minute of second-half injury time to give the "Xolos" the needed margin of victory before a partisan crowd at Estadio Caliente.
Colombian forward Duvier Riascos scored his club leading ninth goal of the season in 68th and Ecuadoran midfielder Fidel Martinez gave Tijuana the lead in the 42nd.
In Toluca, Miguel Layun scored his first goal of the season for America in the 16th and Daniel Montenegro added another in the 37th to pull America equal on aggregate 2-2 following Thursday's first-leg loss.
But Edgar Benitez restored the advantage for Toluca in the 59th and it held on as America ended its day when Juan Medina was ejected in the fifth minute of second-half injury time.
Toluca and Tijuana become the fifth and sixth teams to assure themselves of places in the 2013-2014 Champions League, joining U.S. sides Sporting Kansas City, the San Jose Earthquakes, the Houston Dynamo and LA Galaxy.