The Puerto Rico Islanders also won a second straight CFU Club Champions Cup, getting a pair of goals from Jonathan Fana in extra time to subdue Tempete FC of Haiti 3-1 in the second game of a doubleheader.
The Islanders, which won 3-1 in extra time over Alpha on Wednesday to become one of only three teams to qualify for the Champions League for the fourth straight year, will play Salvadoran side Isidro Metapan in the Preliminary Round.
Tempete will face Mexican Clausura runner-up Morelia while Alpha's victory earned it a spot against Costa Rica's Herediano.
The only berth still undecided is the Nutrilite Canadian Championship winner. Following a 1-1 draw in the first meeting, Toronto FC will host the Vancouver Whitecaps in a second leg replay on July 2 after Wednesday's first attempt was abandoned due to weather.
Dwight Peters gave Alpha United the lead in the 52nd minute then converted one of the penalties to give the Guyanese club the victory. Richard Roy equalized in the 58th minute for Defence Force, which was hurt by the expulsion on Kurt Williams in the first minute of second-half injury time with his second yellow card.
In the championship game, Jay Needham gave Puerto Rico the lead in the 24th minute, but Junior Charles would eventually side the match into injury time with his tying goal in the 42nd.
The Caribbean results give Haiti and Guyana their first teams in the Champions League and means Trinidad will not have at least one club in the four years of the competition.